Guidescan2

GuideScan2 is a tool for genome-wide CRISPR guide RNA (gRNA) design
and analysis in custom genomes. A complete description and usage
information can be found in our manual.pdf. If
you found this tool useful for your research, please cite us at:

@article{schmidt2025genome,
  title={Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2},
  author={Schmidt, Henri and Zhang, Minsi and Chakarov, Dimitar and Bansal, Vineet and Mourelatos, Haralambos and S{\'a}nchez-Rivera, Francisco J and Lowe, Scott W and Ventura, Andrea and Leslie, Christina S and Pritykin, Yuri},
  journal={Genome Biology},
  volume={26},
  number={1},
  pages={1--25},
  year={2025},
  publisher={Springer}
}